Introduction of gene or drug into cells has been used for a wide range of medical applications. We are studying the use of physical delivery of drug or reprogramming factors into the cells and tissue, as a safe and reliable method. We are also exploring integrated diagnostic and therapeutic (theranostics) modalities/nanoparticles/agents, as a unique approach in nanomedicine.
Physical deliveries are based on applying electrical/mechanical stresses to the cells. In this respect, attention has been made to understand biophysical reactions to reversibly manipulate the cells by the external stress. We have been investigating promising physical delivery methods including: electroporation with nanosecond pulsed electric fields, needle-free painless microinjection, micro/nano-particle carrier laser-biolistic delivery, sonoporation with microfluidics, and shock waves; which are shown to be appropriate for clinical applications.
Mona Pakdel*, Zahra Moosavi-Nejad, Rouha Kasra Kermanshahi, Hamid Hosano, Self-assembled uniform keratin nanoparticles as building blocks for nanofibrils and nanolayers derived from industrial feather waste,Journal of Cleaner Production, 335 130331-130331, Feb, 2022, https://doi.org/10.1016/j.jclepro.2021.130331 *Joint publication with Internship student*
Nushin Hosano, Zahra Moosavi-Nejad, Makoto Satoh, Hamid Hosano, Shock Waves Enhance Expression of Glycosphingolipid Tumor Antigen on Renal Cell Carcinoma: Dynamics of Physically Unmasking Hidden Intracellular Markers Independent of Gene-Signaling Pathways, Biomedicines, 10(3) 545-545, Feb, 2022. https://doi.org/10.3390/biomedicines10030545
Tomohiko Yamashita, Reon Yamashita, Hamid Hosano, Takashi Sakugawa, Influence of electrode arrangement on recycling metal-coated plastic (DVD-R) using pulsed electric discharge, Journal of Electrostatics, 110 103557-103557, 2021. https://doi.org/10.1016/j.elstat.2021.103557
M. Thorat, S. Sahu, V. Menezes, A. Gokhale, H. Hosano, Shock loading of closed cell aluminum foams in the presence of an air cavity, Applied Sciences, 10(12), 4128, June 2020 DOI: 10.3390/app10124128
F. Zare, N. Ghasemi, N. Bansal, G. Abhishek, H. Hosano, Increasing the production yield of white oyster mushrooms with pulsed electric fields, IEEE Trans. Plasma Science, 49(2), 805-812, February 2021
M. Sato, T. Sakugawa, T. Yamashita, N. Hosano, H. Hosano, Effects of Voltage and Current Waveforms on Pulse Discharge Energy Transfer to Underwater Shock Waves for Medical Applications, IEEE Trans. Plasma Science, 48(7): 2639-2645, July 2020
Z. Yang ; H. Cao ; J. Hao ; H. Hosano ; S. Katsuki ; H. Akiyama ; C. Zhang, Post-breakdown dielectric recovery characteristics of water for high-repetition-rate switch, IEEE Transactions on Dielectrics and Electrical Insulation 27(3):909-914, June 2020