Research Clusters

Digital Plant Cell Biology

Research Keyword: Bioimage Analysis, Cytoskeleton, Organelle, Plant Cell

With the recent advances in bioimaging equipment such as microscopes, information processing of bioimages is attracting attention as a new research field in bioinformatics. In the IROAST Research Cluster "Digital Plant Cell Biology," we will develop and validate microscopic image analysis techniques to quantitatively evaluate the dynamics of intracellular structures in plant cells. In particular, we will develop biological image analysis frameworks that quantitatively assess the multidimensional biological features of cytoskeleton, and utilize machine learning to make biological discoveries.

Digital Plant Cell Biology

Cluster members

  • Japan
    Takumi HIGAKI Website
    Professor, Faculty of Advanced Science and Technology (FAST), Kumamoto University
  • U.S.A.
    Bo LIU Website
    Professor/ IROAST Visiting Professor, University of California at Davis
  • Japan
    Kae AKITA Website
    Assistant Professor, Kitasato University



Takatsuka H, Higaki T, Ito M (2023) At the nexus between cytoskeleton and vacuole: How plant cytoskeletons govern the dynamics of large vacuoles. Int J Mol Sci 24: 4143.

Morotomi-Yano K, Hiromoto Y, Higaki T, Yano KI (2022) Disease-associated H58Y mutation affects the nuclear dynamics of human DNA topoisomerase IIβ. Sci Rep 12: 20627.

Yoshida D, Akita K, Higaki T (2022) Machine learning and feature analysis of the cortical microtubule organization of Arabidopsis cotyledon pavement cells. Protoplasma in press.

Hotta T, McAlear TS, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, Ohi R (2022) EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. Curr Biol in press.

Takihara Y, Higaki T, Yokomizo T, Umemoto T, Ariyoshi K, Hashimoto M, Sezaki M, Takizawa H, Inoue T, Suda T, Mizuno H (2022) Bone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells. Commun Biol in press.
*This is a result of the IRCMS-IROAST Joint Research Group, in collaboration with Dr. Mizuno and others at IRCMS, as well.

Sakamoto T, Sakamoto Y, Grob S, Slane D, Yamashita T, Ito N, Oko Y, Sugiyama T, Higaki T, Hasezawa S, Tanaka M, Matsui A, Seki M, Suzuki T, Grossniklaus U, Matsunaga S (2022) Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins. Nat Plants in press.

Hotta T, Lee YR, Higaki T, Hashimoto T, Liu B (2022) Two Kinesin-14A motors oligomerize to drive poleward microtubule convergence for acentrosomal spindle morphogenesis in Arabidopsis thaliana. Front Cell Dev Biol. 10:949345. 


Takumi Higaki, Grant-in-Aid for Transformative Research Areas (The Japan Society for the Promotion of Science), Platforms for Advanced Technologies and Research Resources "Advanced Bioimaging Support," "Image Analysis Support," April 2022-March 2028.

Takumi Higaki (Lead Joint Researcher), CREST (Japan Science and Technology Agency), Development of technology platforms for quantitative analysis of spatiotemporal multicellular interaction, "Development of novel research strategy that integrates low-dimensionalized bioinformation with multilevel modeling," October 2021-March 2027.

Takumi Higaki, Grant-in-Aid for Scientific Research (B) (The Japan Society for the Promotion of Science), "Multi-dimensional bioimage analyses with machine learning to reveal the dynamics of membrane vesicles and microtubules in stomatal guard cells," April 2020-March 2023.



The International Symposium on "Plant-Structure-Optimization" in Nara (Nov 19-20, 2022)


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