Publications

2025

• Morphological and transcriptome analysis of the near-threatened orchid Habenaria radiata with petals shaped like a flying white bird

  Takeda S, Nishikawa Y, Tachibana T, Higaki T, Sakamoto T, Kimura S

  Plants 14(3): 393, 2025

  https://doi.org/10.3390/plants14030393

• Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures

  Manami Ichita, Haruna Yamamichi, and Takumi Higaki*

  Plant Molecular Biology 115, 29 (2025)

  https://link.springer.com/article/10.1007/s11103-025-01558-w

  *The results of this research has been released to the media as a press release on January 31, 2025.

2024

• Deep learning-based cytoskeleton segmentation for accurate high-throughput measurement of cytoskeleton density

  Ryota Horiuchi, Asuka Kamimura, Yuga Hanaki, Hikari Matsumoto, Minako Ueda, and Takumi Higaki*

  Protoplasma, in press

  https://doi.org/10.1007/s00709-024-02019-9

  *The results of this research has been released to the media as a press release on September 19, 2024.
  *Supported by "IROAST Proof Reading Support Programs FY2024"
  

• Machine learning accelerates screening of osteoclast differentiation inhibitors from natural products

  Hitora Y, Hokaguchi M, Sadahiro Y, Higaki T, Tsukamoto S

  J Nat Prod. 87(10): 2393–2397 (2024)
  *Added the volume number on Feb. 3, 2025
  

  https://pubs.acs.org/doi/10.1021/acs.jnatprod.4c00640

• The PATROL1 function in roots contributes to the increase in shoot biomass

  Notaguchi M, Ichita M, Kawasoe T, Monda K, Kurotani K, Higaki T, Iba K, Hashimoto-Sugimoto M

  Planta, 260, 5, 105 (2024)

  https://doi.org/10.1007/s00425-024-04526-8

• HD-ZIP IV genes are essential for embryo initial cell polarization and the radial axis formation in Arabidopsis

   Sayuri Tanaka, Yuuki Matsushita, Yuga Hanaki, Takumi Higaki, Naoya Kamamoto, Katsuyoshi Matsushita, Tetsuya Higashiyama, Koichi Fujimoto, and Minako Ueda*

  Current Biology, 34(20): 4639–4649 (2024)
  *Added the volume number on Feb. 3, 2025

  https://doi.org/10.1016/j.cub.2024.08.038

  *The results of this research has been sent out to the media as a press release on September 18, 2024 and released on September 20, 2024.

• Precocious cell differentiation occurs in proliferating cells in leaf primordia in Arabidopsis angustifolia3 mutant

  Ezaki K, Koga H, Takeda-Kamiya N, Toyooka K, Higaki T, Sakamoto S, Tsukaya H

  Front Plant Sci 15 (2024): 1322223

  https://doi.org/10.3389/fpls.2024.1322223

• Neopetromin, a Cyclic Tripeptide with a C–N Cross-Link, from the Marine Sponge Neopetrosia sp., That Causes Vacuole Fragmentation in Tobacco BY-2 Cells

  Yuki Hitora, Ahmed H. El-Desoky, Yusaku Sadahiro, Ai Sejiyama, Amiri Kinoshita, Yuji Ise, Esther D. Angkouw, Remy E. P. Mangindaan, Takumi Higaki, and Sachiko Tsukamoto*

  Journal of Natural Products, 87 (4), 1197-1202 (2024)

  https://doi.org/10.1021/acs.jnatprod.4c00158

2023

• Comprehensive and quantitative analysis of intracellular structure polarization at the apical–basal axis in elongating Arabidopsis zygotes

  Yukiko Hiromoto, Naoki Minamino, Suzuka Kikuchi, Yusuke Kimata, Hikari Matsumoto, Sakumi Nakagawa, Minako Ueda and Takumi Higaki

  Sci Rep 13, 22879 (2023)

  https://doi.org/10.1038/s41598-023-50020-8

• Intracellular trafficking regulation of plasma membrane H+-ATPase and environmental response in plants

  Ichita M, Higaki T

  Cytologia, 88: 169-173. (Published: 25 Sep 2023) 

  https://doi.org/10.1508/cytologia.88.169

• The SYP123–VAMP727 SNARE complex delivers secondary cell wall components for root hair shank hardening in Arabidopsis

  Hirano T, Ebine K, Ueda T, Higaki T, Watanabe-Nakayama T, Konno H, Takigawa-Imamura H, Sato MH

  Plant Cell. 35(12), 4347-4365.  (Published 15 Sep 2023)

  https://doi.org/10.1093/plcell/koad240

• Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice

  Yue Y, Hotta T, Higaki T, Verhey KJ, Ohi R 

  Curr Biol,  33(19) 4111-4123. e7 (Published 9 Oct 2023)

  https://doi.org/10.1016/j.cub.2023.07.062

• Smooth elongation of pavement cells induced by RIC1 overexpression leads to marginal protrusions of the cotyledon in Arabidopsis thaliana

  Kikukawa K, Takigawa-Imamura H, Soga K, Kotake T, Higaki T 

  Plant and Cell Physiology, 64, 11, 1356–1371, 2023 (Published 18 Sep, 2023)

  https://doi.org/10.1093/pcp/pcad094

  *Supported by "IROAST Proofreading/Publication Support Program 2022"
  

• Arabidopsis thaliana Subclass I ACTIN DEPOLYMERIZING FACTORs Regulate Nuclear Organization and Gene Expression

  Matsumoto T, Higaki T, Takatsuka H, Kutsuna N, Ogata Y, Hasezawa S, Umeda M, Inada N 

   Plant Cell Physiol, 64(10), 1231-1242.  (Published: 18 Aug 2023) 

  https://doi.org/10.1093/pcp/pcad092

• Cell polarity linked to gravity sensing is generated by LZY translocation from statoliths to the plasma membrane

  Takeshi Nishimura, Shogo Mori, Hiromasa Shikata, Moritaka Nakamura, Yasuko Hashiguchi, Yoshinori Abe, Takuma Hagihara, Hiroshi Y. Yoshikawa, Masatsugu Toyota, Takumi Higaki, Miyo Terao Morita

  Science, 381, 6661, pp1006-1010 (2023)

  https://doi.org/10.1126/science.adh9978

• Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells

  T Wint H, Li J, Abe T, Yamada H, Higaki T, Nasu Y, Watanabe M, Takei K, Takeda T 

   J Cell Sci. 136, 10, jcs.260827. (Published: 31 May 2023)

  https://doi.org/10.1242/jcs.260827

• At the nexus between cytoskeleton and vacuole: How plant cytoskeletons govern the dynamics of large vacuoles

  Takatsuka H, Higaki T, Ito M 

  Int J Mol Sci 24: 4143

  https://www.mdpi.com/1422-0067/24/4/4143

• Machine learning and feature analysis of the cortical microtubule organization of Arabidopsis cotyledon pavement cells

  Yoshida D, Akita K, Higaki T

  Protoplasma, 260, 3, 987-998, 2023

  https://doi.org/10.1007/s00709-022-01813-7

2022

• Disease-associated H58Y mutation affects the nuclear dynamics of human DNA topoisomerase IIβ

  Morotomi-Yano K, Hiromoto Y, Higaki T, Yano KI 

  Sci Rep 12: 20627 (2022)

  https://www.nature.com/articles/s41598-022-24883-2

• EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules

  Hotta T, McAlear TS, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, Ohi R

  Curr Biol, 32, 18, 3898-3910.e14 (2022)

  https://doi.org/10.1016/j.cub.2022.07.027

•  Bone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells

  Takihara Y, Higaki T, Yokomizo T, Umemoto T, Ariyoshi K, Hashimoto M, Sezaki M, Takizawa H, Inoue T, Suda T, Mizuno H 

  Commun Biol, 5, 776 (2022)

  https://doi.org/10.1038/s42003-022-03733-x

  *This is a result of the IRCMS-IROAST Joint Research Group, in collaboration with Dr. Mizuno and others at IRCMS, as well.

• Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins

  Sakamoto T, Sakamoto Y, Grob S, Slane D, Yamashita T, Ito N, Oko Y, Sugiyama T, Higaki T, Hasezawa S, Tanaka M, Matsui A, Seki M, Suzuki T, Grossniklaus U, Matsunaga S 

  Nat Plants, 8, 940-953 (2022)

  https://doi.org/10.1038/s41477-022-01200-3

• Two Kinesin-14A Motors Oligomerize to Drive Poleward Microtubule Convergence for Acentrosomal Spindle Morphogenesis in Arabidopsis thaliana

  Hotta T, Lee YR, Higaki T, Hashimoto T, Liu B

  Front Cell Dev Biol. 10 (2022):949345.

  https://doi.org/10.3389/fcell.2022.949345