Ruda LEE

Dr. Ruda LEE

Associate Professor, Institute of Industrial Nanomaterials (IINa)

Research Fields
Drug delivery system/Molecular imaging/Hybrid particles/Multi-drug resistance/Biomedical Engineering/Environmental Science 

Research interest

Research in the Lee lab develops novel molecular imaging sensors and multifunction drug delivery carriers for ‘Theragnosis’ (Therapy + Diagnosis).

1. Enzyme specific activated imaging sensor based real-time in vivo imaging

Proteases are among the most studied enzyme families due to their involvement in regulating diverse disease processes and their potential value as biomarkers and therapeutic targets. We reported various protease target Near-infrared fluorescence (NIRF) imaging sensors and detecting pathologic processes at the cellular and molecular levels in vivo, such as cancer, arthritis, brain disorder, and inflammation.


2. Active targeting drug delivery nanomaterials

Active targeted drug delivery material delivers a therapeutic and diagnostic agent to a targeted diseased area. Drug-loaded nanomaterials (NPs) are decorated by the peptide, aptamer, and antibody for recognition by specific receptors/antigens on target cells. In this strategy, NPs can avoid non-specific biodistribution, reducing cytotoxicity and treatment dosage. It could provide patients to have a chance for effective treatment.


3. Stimuli responsive controlled drug release system

Owing to precise stimuli response, stimuli-responsive DDSs can control drug release to improve the curative effects, reduce the damage to normal tissues and organs, and decrease the side effects of traditional anticancer drugs. We designed endogenous stimuli-responsive materials (pH, enzyme, and redox responsive) and exogenous stimuli-responsive materials (such as light and ultrasound)

Professional Education

Ph.D., Korea University, School of Medicine (2013)

Research Training

2022-present Associate Professor (IINa, Kumamoto University, Japan)
2017-2021 Associate Professor (IROAST, Kumamoto University, Japan)
2014-2016 Postdoctral fellow (IIT, Italy)
2013-2014 Postdoctral fellow (KIST, Korea)
2009-2013 MS & Ph.D course trainee (KIST, Korea)
2008-2009 Research Assistant (KIST, Korea)

Selected peer-reviewed publications

  1. Lee A et al. Early diagnosis of arthritis in mice with collagen-induced arthritis, using a fluorogenic MMP 3-specific polymeric probe. Arthritis Rheum 2011
  2. Lee A et al. Measurement of MMP Activity in Synovial Fluid in Cases of Osteoarthritis and Acute Inflammatory Conditions of the Knee Joints Using a Fluorogenic Peptide Probe-Immobilized Diagnostic Kit. Theranostics 2012
  3. Lee A et al. A novel near-infrared fluorescence chemosensor for copper ion detection using click ligation and energy transfer. Chem Comm. 2013
  4. Lee A et al. TNF-α Gene Silencing Using Polymerized siRNA/Thiolated Glycol Chitosan Nanoparticles for Rheumatoid Arthritis. Mol Ther 2014
  5. Lee A et al. Spherical Polymeric Nanoconstructs for Combined Chemotherapeutic and anti-Inflammatory Therapies. Nanomedicine : NBM 2016
  6. Lee A et al. Dexamethasone-loaded nanoconstructs for treating and monitoring inflammation in murine colitis models. Theranostics 2017
  7. Lee A et al. Visualization of MMP-2 Activity Using Dual-Probe Nanoparticles to Detect Potential Metastatic Cancer Cells. Nanomaterials 2018
  8. Kim MW et al. Anti-EGF Receptor Aptamer-Guided Co-Delivery of Anti-Cancer siRNAs and Quantum Dots for Theranostics of Triple-Negative Breast Cancer. Theranostics 2019 (co-author)
  9. Ueno S et al. Development of ErbB2-Targeting Liposomes for Enhancing Drug Delivery to ErbB2-Positive Breast Cancer. Pharmaceutics 2020 (Corresponding)
  10. Park YI et al. pH-sensitive multi-drug liposomes targeting folate receptor β for efficient treatment of non-small cell lung cancer.Journal of Controlled Release 2021 (Corresponding)

Lab members

-  Associate Prof.         Ruda Lee
-  Students M2  Miu Goto                    
M2 Nozomi Koga
M2 Naoya Iwata   
M2 Ren Yamamoto           
M1 Mahiro Morita
M1 Yoshihito Hirata  
M1 Tsugumi Araki                                             



  1. FY2022 JSPS, Grant-in-Aid for Scientific Research (C)
  2. 令和4年度「クロス・フロンティア研究推進事業」, 熊本大学
  3. Brain Pool Korea 2022
Book publications

Ruda Lee, Yong Il Park. Exosome detection for early cancer diagnosis. Springer Handbook of Cancer and Immunology, Sep 2023

  1. Ruda Lee, Emerging Role of Nanotechnology for Water Sustainability. Korea International Water Week (KIWW) 2022. 2022/11/24, Daegu, Korea. 

    (Dr. Ruda LEE in the center)                               (Group Photo at the venue)

  2. Ruda Lee, Nanoplatform for constructing new approaches to cancer treatment. Kangwon National University, 2022/03/30, Web.


  • Japan Agency for Medical Research and Development (AMED), FY 2021 Infectious Diseases and Immunology Research: U.S.-Japan Cooperative Medical Sciences Program Collaborative Awards, "Investigating DNA origami vaccine efficacy in infectious diseases", April 2021-March 2023

  • Grant-in-Aid for Early-Career Scientists (The Japan Society for the Promotion of Science), "Overcoming multi drug resistance in 3D breast cancer cell model by pH-sensitive biomimetic nanoparticles," April 2020-March 2022.

  • Kumamoto University Support Program for enhancing Research Ability of Women Researchers FY2020 (正式名:令和2年度熊本大学女性研究者研究力向上のための支援事業),"Development of stimuli responsive high drug-loading nanoparticles", 2020.

  • Kumamoto University Research Achievement Award FY2023 (令和5年度国立大学法人熊本大学研究業績表彰), Nov. 27, 2023

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